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Expr11922
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gcn-1 was expressed in most cells during all stages of development. gcn-1 expression was observed in head neurons, hypodermal cells, intestinal cells, body wall muscles, and pharyngeal neurons, including the M4 neuron. |
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Expr15558
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Expr9325
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Synaptogyrin is expressed in all 26 GABAergic neurons including also RMER and most though not all other neurons. Synaptogyrin is absent in amphids and phasmids and can be detected in non-neuronal glial-like sheath cells in adult worms. The cephalic neurons CEPDR/L and CEPVR/L and amphid-associated sheath cells CEPshDR/L, CEPshVR/L were tentatively positive. Several other neurons that could be tentatively identified in the anterior part are MI, M4, I4, AVL, AIY, RIS, I5, M3R/L, and in the posterior part DVA, AS11, ALNR/L, DVC, DVB, PQR, DA9 (characteristic axonal process denoted by arrowhead), VD13, DD6, VD12. Of these, AVL, RIS, VD13, DD6 and VD12 are GABAergic based on the colocalization with the unc-47p::GFP reporter. In addition, IL neurons were tentatively identified in the anterior (IL*). Synaptogyrin reporter constructs are also expressed in developing neurons. The expression of sng-1p::YFP is closely associated with the development of the nervous system being absent in the gastrula stage with first fluorescence in neuronal precursor cells and newly-formed neurons in the anterior part during the 1.5-fold stage. In addition, it is also detected transiently in cells in the posterior body at the 1.25-fold and 1.5-fold stage. |
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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F22A3.3 = glr-8 |
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Expr823
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I1, I2, MC, NSM, M3 (left/right), I3, MI M4, M3 (left/right), I6, M5, BDU, ALM, PLM, URB (left/right) |
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Expr15583
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Expr15586
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Expr15651
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Expr15652
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Expr15589
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Expr13158
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Expr15591
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Expr12196
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To determine where lite-1 is expressed and identify likely sites of lite-1 function, the authors generated and examined worms carrying one of four transgenes derived from the wild-type lite-1 locus: a genomic fragment (lite-1 genomic), a transcriptional fusion (lite-1prom::gfp), a C-terminal translational fusion (lite-1prom::lite-1::gfp), and an N-terminal translational fusion (lite-1prom::gfp::lite-1). GFP expression was observed in a total of 29 cells: pharyngeal neurons M1, M4, M5, and MI; non-pharyngeal neurons ASK, ADL, ASI, ASH, AVG, AVB, RIM, ADF, PHA, PHB, and PVT; and non-neuronal cells Hyp3 (hypoderm), AMso (amphid socket cells), and PHso (phasmid socket cells). AVB was observed only with the C-terminal fusion transgene, and RIM and ADF were observed only with the transcriptional fusion transgene. lite-1 expression in AVG and PVT was previously reported. |
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Expr15598
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Expr11622
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Expression of ceh-34::gfp transgene began during embryogenesis. CEH-34::GFP was localized to the nuclei of expressing cells. During embryonic morphogenesis and larval development and throughout adulthood, expression of the ceh-34::gfp transgene was seen predominantly in pharyngeal cells. The ceh-34::gfp transgene was expressed in all pharyngeal neurons (M4, I1, MI, I3, M3, NSM, MC, I2, I4, I5, I6, M1, M2, and M5), some pharyngeal muscle cells (pm1 and pm2) and pharyngeal epithelial cells (e1 and e3), and some body wall muscles around the anterior pharynx. |
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Expr15604
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Expr14590
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Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
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Expr15608
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Expr15369
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Expr15611
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Expr3759
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ser-7::gfp DNA injected into C. elegans at 0.5-0.05 ng/Ul produced lines that exhibited marked GFP fluorescence in a number of pharyngeal neurons, including the MCs, M4, I2s, I3, M5 and occasionally in the M3s, I4, I6 and M2s. Strong GFP fluorescence was also observed in the vulval muscles. |
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Expr15436
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unc-17(3k) |
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Expr14243
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mpz-1 was identified as being expressed in the pharyngeal neurones (including M4 and NSM based on their size and relative position), extrapharyngeal neurones, body wall muscle and vulval muscle. Co-expression of mpz-1::mRFP-1 with mgl-1::GFP was identified in neurones of the nerve ring and the pharyngeal nervous system in the desheathed pharynx preparation. |
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Expr2628
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Expression was detected in a few nuclei in the head as the embryo reached morphogenesis stage at ~300 minutes of development. Expression was identified in more and more nuclei as development proceeded, so that by the 1 1/2-fold stage a large number of neuronal cells in the head and a few cells in the pharynx expressed zag-1::YFP. At this stage expression was also prominent in motorneurons in the ventral cord and in neurons in tail ganglia. Expression was maintained during the 3-fold stage, but reduced to undetectable levels in most cells before hatching, when only a few cells in the head still expressed zag-1::YFP. In the L1/L2 stage expression was detected transiently in postembryonic motorneurons. Expression was maintained in a few head neurons throughout the entire life cycle. Pzag-1::YFP construct was expressed predominantly in neurons in head and tail ganglia, starting approximately midway through embryogenesis. In some of these neurons expression was maintained throughout development. Additional expression was found consistently in the intestinal and anal depressor muscles during all life stages as well as occasionally in body-wall muscles during embryogenesis. |
zag-1::YFP signal was detected only in the nuclei of cells. |
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Expr14587
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The integrated smoc-1 transcriptional reporter showed strong GFP expression. GFP was first detectable in several cells located in the anterior of bean stage embryos. In the developing larvae, GFP is expressed in cells of the pharynx, the intestine and the posterior hypodermis. Pharyngeal cells expressing smoc-1p::gfp include the epithelial cells e2, the marginal cells mc1 and mc2, the M4 neuron, and all six of the pharyngeal/intestinal valve cells. Cells of the posterior hypodermis expressing smoc-1p::gfp include hyp8, hyp9, hyp10, and hyp11. Expression in these tissues persisted from the L1 larval stage through adulthood. We noted that while all transgenic animals showed GFP expression in the pharynx and the posterior hypodermis, a small fraction of animals (~8%) did not exhibit GFP expression in all or some of the intestinal cells. We observed no GFP expression in any other tissues, including the nerve cord, body wall muscles (BWMs), or the M lineage. |
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Picture: Figure 3A to 3G. |
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Expr7888
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In all lines, GFP expression was observed exclusively in the M4 pharyngeal neuron from the 2-fold stage [~450 min post first cleavage (pfc)] through adulthood. In earlier embryos prior to pharyngeal formation, strong and reproducible GFP was also observed in a cell called ABalaappa beginning at 172 min pfc and continuing in the daughters of this cell. The anterior daughter of this cell undergoes pro- grammed cell death, whereas the posterior daughter (called ABalaappap) continues to express GFP until at least 350 min pfc, before dividing to produce the AVAR ventral cord interneuron and the OLQsoVR socket cell. Weak and variable expression was also observed in other cells including ABalapap(a/p). |
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50-70 cell embryo(author) = 51-cell embryo(curator). early embryo(author) = blastula + gastrulating embryo(curator). fragment altered 7/97, at request of IHope late embryo(author) = 2-fold embryo(curator). life_stage summary : each cell-group has different pattern |
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Expr21
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The last expression component to appear is in certain cells of the somatic gonad. The D-cells of the vulval labia and unidentified cells of the spermathecal structures begin expression in L4, whilst gonadal morphogenesis is ongoing. The D-cells do not express beyond the first oocyte fertilisations (no zygotes are usually visible when these cells are stained), the spermathecal staining lasting slightly longer into adulthood The next stage at which expression is evident is during the elongation phase of late embryogenesis when the worm is approximately 2 fold. The nuclei of the M2 motor neurones in the terminal bulb of the pharynx stain strongly. More pharyngeal cells show expression as morphogenesis proceeds until at hatching the two I1 interneurones of the metacorpus, either the e2 or m2 cells of the procorpus, and the m8 muscle cell at the pharyngeal-intestinal boundary can all be seen. This pattern remains through the rest of the life cycle, although the m8 expression is lost during early larval stages These early larval stages also see the appearance of expression in the tail region. The nuclei of the anal sphincter cell and 3/4 neuronal cells of the posterior ganglia comprise this regional component of the pattern This gene gives rise to a complicated multicomponent developmental expression pattern. Earliest expression is seen during the cleavage stage of embryogenesis, in the clonal descendants of the E blastomere, the founder cell giving rise the whole of the gut of the adult animal. Expression begins in Ea and Ep just after gastrulation, and continues into each of the granddaughters of these two cells. At this stage, the expressing cells clearly outline the emerging form of the gut. This component ends at about the 150/200 cell stage |
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