WormMine: ExpressionPattern Expr971

• Pattern: The highest and most consistent expression was observed with several independent strains derived from the largest expression construct (DYNGFP1B-7). Expression was nearly exclusive to neurons which possessed ciliated sensory specializations. The extent and level of expression correlated roughly with the growth of the ciliated endings of these neurons. Since neurulation (and presumably cilia growth) occurs in ovo, and since cells at this stage of development are compact and difficult to identify, the authors did not demonstrate that all ciliated neurons express the fusion construct. However, a subset of neurons with ciliated endings (ADL, ASH, ASI, ASJ, PHA, PHB, AQR, and PQR) generally show modest and somewhat variable expression of the fusion construct throughout the life span of the animal. Expression was first noted at approximately 380 - 400 min after fertilization-a time corresponding to the comma stage of development in two cells flanking the developing pharynx. Expression rapidly increased in extent and intensity, peaking in the 3-fold stage in many cells (>30) located anterior and posterior to the nerve ring, as well as in four cells in the developing tail. Expression in the region adjacent to the nerve ring was consistent with all ciliated neurons in this region showing fluorescence. Expression was maintained through most of embryogenesis but decreased in extent and intensity shortly after hatching; late L1 larvae typically showed expression in the phasmids (PHA and PHB) and five or six neuron classes in the head, which usually included ADL, ASK, ASH, ASJ, and ASI but also variably included a subset of ASE, ASG, ADF, AFD, FLP, and ADE. With occasional exceptions, most of the neuron classes anterior to the nerve ring, which based on position may have been BAG, IL1, IL2, OLL, CEP, and OLQ, ceased expressing the reporter late in embryonic development. The neurons with the most elaborate ciliated endings-the amphid wing cells, AWA, AWB, and especially AWC-did not show expression after hatching. Expression did not decrease much further than this as the animal aged. Both these cells showed expression in developing larvae from the L3 stage on. The postdeirid structure in the worm's midbody region also appears postembryonically and contains a ciliated neuron (PDE) that showed clear fluorescence in many animals. This expression was also transiently restricted to a period of time shortly following the appearance of this neuron. In addition, two examples of weak expression in unciliated cells were noted. First, at least one pharyngeal neuron (tentatively identified as I4) showed weak expression in some animals. Finally, barely perceptible fluorescence was occasionally observed in the coelomocytes of some worms.
• Primary Identifier: Expr971

31 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Neuron class of one sensory neuron, basal body, not part of a sensillum, projects into preanal gangion.	PQR	lineage name: QL.ap	WBbt:0004096
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASEL	lineage name: ABalppppppaa	WBbt:0003904
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASER	lineage name: ABpraaapppaa	WBbt:0003903
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASGL	lineage name: ABplaapapap	WBbt:0003892
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASGR	lineage name: ABpraapapap	WBbt:0003891
Neuron, dopaminergic of postderid sensillum	PDEL	lineage name: V5L.paaa	WBbt:0004383
Neuron, dopaminergic of postderid sensillum	PDER	lineage name: V5R.paaa	WBbt:0004382
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751
Amphid neuron, prob. chemosensory	ADFL	lineage name: ABalpppppaa	WBbt:0004003
Amphid neuron, prob. chemosensory	ADFR	lineage name: ABpraaappaa	WBbt:0003999
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASIR	lineage name: ABpraapapppa	WBbt:0003887
Amphid neuron, prob. chemosensory	ADLL	lineage name: ABalppppaad	WBbt:0003997
Amphid neuron, prob. chemosensory	ADLR	lineage name: ABpraaapaad	WBbt:0003995
one member of amphid neurons, single ciliated ending, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASIL	lineage name: ABplaapapppa	WBbt:0003888
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASKL	lineage name: ABalpppapppa	WBbt:0003884
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASKR	lineage name: ABpraaaapppa	WBbt:0003883
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASHL	lineage name: ABplpaappaa	WBbt:0003890
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASHR	lineage name: ABprpaappaa	WBbt:0003889
Phasmid neurons, probably chemosensory	PHAL	lineage name: ABplpppaapp	WBbt:0004366
Phasmid neurons, probably chemosensory	PHAR	lineage name: ABprpppaapp	WBbt:0004365
Phasmid neurons, probably chemosensory	PHBL	lineage name: ABplapppappp	WBbt:0004364
Phasmid neuron, probably chemosensory	PHBR	lineage name: ABprapppappp	WBbt:0005659
Anterior deirid, sensory neuron, dopaminergic	ADEL	lineage name: ABplapaaaapa	WBbt:0004009
Anterior deirid, sensory neuron, dopaminergic	ADER	lineage name: ABprapaaaapa	WBbt:0004007
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJL	lineage name: ABalpppppppa	WBbt:0003886
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJR	lineage name: ABpraaappppa	WBbt:0003885
Neuron class of one sensory neuron, basal body; not part of a sensillum, projects into ring.	AQR	lineage name: QR.ap	WBbt:0003927
Neuron, ciliated ending in head, no supporting cells, associated with ILso	FLPL	lineage name: ABplapaaapad	WBbt:0004798
Neuron, ciliated ending in head, no supporting cells, associated with ILso	FLPR	lineage name: ABprapaaapad	WBbt:0004797

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000485	che-3	F18C12.1	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002