WormMine: AnatomyTerm WBbt:0005038

• Definition: Ring motor neuron/interneuron, many synapses
• Name: RMDDR
• Primary Identifier: WBbt:0005038
• Synonym: lineage name: ABarappapaa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABarappapaa	ABarappapaa nucleus		WBbt:0002722

2 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts enriched in RMD_DV according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:RMD_DV_enriched
	Top 300 transcripts enriched in RMDL, RMDR, RMDDL, RMDDR, RMDVL, RMDVR according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:RMD

76 Expression Patterns

• Primary Identifier: Expr15649

• Primary Identifier: Expr15442

• Primary Identifier: Expr15558

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr12719
• Pattern: acc-1 and acc-2 fosmid reporters show very restricted and non-overlapping expression in the adult nervous system. The acc-1 fosmid reporter is expressed in a subset of cholinergic neurons, including cholinergic neurons in the ventral nerve cord, the retrovesicular ganglion and a few head neurons (including the SMD, RMD motor neurons, the AVA and AVE command interneurons and the SAA neurons). A small number of glutamatergic neurons also express acc-1 (including the pharyngeal neurons MI and M3, the PLM neurons and an unidentified neuronal pair in the lateral ganglion).

• Primary Identifier: Expr8850
• Remark: Picture: Fig 3.
• Pattern: Neuronal Expression: AVA, AVB, AVE, PVC, AIB, AUA, AVG, RIB, RIC, SAA, SIA, SIB, RIF, RIM, RMD, RME, SMD, DA, DB, VA, VB, M5, NSM, MC, I3, MI?. Non-neuronal Expression: rectal epithelium, body wall muscle, spermethecae, vulva muscle.

• Primary Identifier: Expr15604

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr249
• Pattern: AVG AVJ DVC PVC PVQ RIG RIS RMD RMEL/R SMD URY [Nature 378:82]

• Primary Identifier: Expr15371

• Primary Identifier: Expr15406

• Primary Identifier: Expr14126
• Pattern: RMDV, RMDD, sometimes other dim neurons, pharynx (sometimes I2 neurons), pharyngeal-intestinal valve, head mesodermal cell, anal depressor muscle, phasmids (dim)

• Primary Identifier: Expr3310
• Pattern: Expression was detected first in neuronal precursor cells at the comma stage. Slightly later strong expression was found in the hypodermal cells. In newly hatched L1 larvae this construct continued to be expressed in neuronal cells in the head and tail, in cells of the ventral nerve cord and of the pharynx. During the early L1 stage hypodermal expression disappeared. The GFP-positive neurons in animals carrying pT14G10.2::GFP-II were identified as the RMDD, RMD, RMDV, SMDD, and SMDV, which are ring motoneurons. Also the more posteriorly located BDU cells and the ALN, PVR, and PVT cells in the tail expressed GFP. Analysis of synchronized worms showed that GFP is present around the time of molting, but is barely detectable during part of the intermolts or in adults. During the outgrowth of the gonadal primordium expression was seen in the distal tip cell and oviduct sheath cells.

• Primary Identifier: Expr320
• Remark: Neuronal gene expression pattern from collation by Shawn Lockery of neuron-specific promotors posted 20/04/98 (http://chinook.uoregon.edu). Since cell AS is listed under 'body' in the pattern, it presumably means the AS.1-11 ventral cord neurons rather than the AS amphid neurons.[sdm-curator]
• Pattern: head: IL2 URA URB SAA SAB SIA SIB SMB SMD RMD AIY, sev more; phar: M1 M2 M5 I1f I6f, others; body: VA VB VC DA DB AS SDQ HSNf; tail: ALN PLN others [J. Duerr (personal communication to Shawn Lockery)], antibody

• Primary Identifier: Expr15648

• Primary Identifier: Expr15423

• Primary Identifier: Expr2382
• Pattern: GFP was predominantly expressed in head neurons, a few cells in the tail and hypodermis except for seam cells. A total of 30-50 fluorescent neurons of varying intensities were observed in the head. Stronger or more consistent expression was observed in RMDV, RMD, SMDV, RIB, ASK and probably RMDD and SMDD neurons. Expression in AWC, ASE, AVJ and ASH was sometimes seen. In the tail, DVB and DVC neurons were probably expressing. GFP was also expressed in ventral cord neurons and intestine, typically near the tail. L2 and L3 larvae show extensive expression most notably in head neurons and hypodermis, and the expression is weaker in L4 and adults. Expression is also seen in later embryos.

• Primary Identifier: Expr862
• Remark: Reporter gene fusion type not specified.
• Pattern: At the L1 larval stage CEH-6ceh-6 is expressed in four pairs of bilaterally symmetric neurons in the lateral ring ganglion of the animal. These neurons are the RMDDLR, RMDVLR, AUALR and AVHLR neurons. The expression in RMDD and RMDV is weaker than in AUA and AVH. ceh-6 is also expressed in the excretory cell, very strongly with the lacZ reporter construct but more weakly with the antibody, which is probably due to the large volume of the nucleus. Despite the nuclear localization signal in the lacZ construct, the beta-galactosidase was occasionally expressed at such high levels that the excretory canals were also stained. Posterior to the excretory cell, the neurons SABVLR in the retro-vesicular ganglion express CEH-6ceh-6. Additional CEH-6-expressing cells in the body and tail were observed only with the antiserum, indicating that the ceh-6 reporters may not contain all promoter elements. In the body region, expression was observed in dividing P.na cells in the ventral nerve cord in L1 animals. The Pn.a expression is transient, appearing before the cell division of Pn.a and fading in the daughter cells. During the cell division, CEH-6 is localized to the cytoplasm of the dividing cells. It appears that the posterior daughters lose CEH-6 before that of the anterior daughter, as seen in the anterior Pn.ap cells. In the tail, CEH-6 expression in L1 animals is seen around the rectum in the five rectal cells B, Y, U, F and K. After K has divided, only the rectal cell K.a expresses CEH-6, though expression during the division was not monitored. At the L2 stage, a sixth cell becomes apparent that, based on its position at the very bottom of the rectum, is deduced to be P12.pa. Head and rectal expression of CEH-6 persists into adulthood. In addition, in adult animals four symmetric CEH-6 expressing cells are seen around the vulva, possibly one set of the vul cells, though did not determine their identity. During embryogenesis ceh-6 is expressed at the comma stage in two clusters one cluster corresponds to the ectodermal cells surrounding the anus (B, Y, U, F and K), and the other cluster corresponded to the cells in the head described above, many of which are located relatively close to each other at that stage in development.

• Primary Identifier: Expr8842
• Remark: Picture: Fig 3.
• Pattern: Neuronal Expression: ASI, ASJ, ASK, AWB, PLM?, AVA, AVE, AIB, AIN?, AIY, AUA, RIC, RIG, DVA?, RMD, RME, SMD, URA?, PDA, DA, DB, DD. Non-neuronal Expression: gut, distal tip cell(embryo, early larval stages), coelomocytes, arcade cells.

• Primary Identifier: Expr12716

11 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016
	The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes.		late cleavage stage embryo Ce	WBls:0000014

5 Parents

Definition	Name	Synonym	Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
ventral ganglion, anterior part.	ventral ganglion (ant)		WBbt:0005266
Neuron class of six major motoneurons, which innervate muscles in the head via NMJs in the nerve ring.	RMD		WBbt:0005400
cell that exists in and is part of an embryo.	embryonic cell		WBbt:0007028
embryonic cell	ABarappapa		WBbt:0006230