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Expression Pattern :

Pattern  DAF-12::GFP was expressed widely in most cells including tissues modified for dauer formation or by stage. It was expressed in phenotypically affected target tissues (e.g., epidermis, vulva, somatic gonad, intestine, pharynx, sex myoblasts), as well as other tissues with no known phenotype (e.g., nervous system, body wall muscle). Expression was seen from embryo to adult, but was most elevated and widespread during L2. Epidermis: In seam cells and hypodermis, DAF-12::GFP expression was first seen at the 3-fold stage of embryogenesis, increased by late L1, peaked during L2, diminished by late L3, and was low or off in L4 and young adults. Expression was also seen in the ventral epidermal L1 P ectoblasts, L2 vulval precursors, and their L3 descendants. Expression continued during L4 vulval morphogenesis and persisted occasionally in the mature adult vulva at reduced levels. Somatic Gonad: Faint expression was seen as early as L1 in Z1 and Z4 somatic gonadal precursors. By L2, their descendants, the somatic gonadoblasts, including the migratory distal-tip cell, strongly expressed DAF-12::GFP. Expression continued in somatic gonadoblast descendants and distal-tip cells in L3 and early L4. In the adult, expression was robust in the mature spermatheca and uterus. Intestine: Expression in intestinal nuclei was diffuse during the larval stages, but became somewhat stronger in the adult. Nervous System: Only a handful of head and tail neurons expressed GFP early in L1. By mid-L2, DAF-12::GFP was expressed strongly throughout the nervous system, including the ventral cord and peripheral neuroblasts. Expression continued in many neurons in the adult, albeit at reduced levels. Musculature: Expression in body wall muscles became visible by late L1 and L2. Expression continued at later larval stages and in the adult at reduced levels. Expression in pharyngeal muscle was strong by L2 and downregulated by adult. DAF-12::GFP was also expressed in the L1 M-mesoblast, and its derivatives, including post-embryonic body wall muscles, sex myoblasts and their descendants. Dauer formation: DAF-12::GFP was downregulated in dauer larvae in all tissues, but perdured in the somatic gonad and occasional neurons. Upon recovery from dauer diapause, DAF-12::GFP was expressed weakly in most tissues. Primary Identifier  Expr1047
Remark  Lineage expression: M lineage. The transgene rescued the Daf-d phenotype of rh61rh411 animals, suggesting that daf-12::GFP is functional. Several integrated transgenic lines were made and all gave a similar pattern of expression. Subcellular Localization  DAF-12::GFP localized primarily to the nucleus, except during mitosis, when expression became diffuse.
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65 Anatomy Terms

Definition Name Synonym Primary Identifier
neuron with cell body associated with the ventral nerve cord. ventral cord neuron ventral cord motoneuron WBbt:0005300
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages. intestine gut WBbt:0005772
female genital. vulva   WBbt:0006748
an accordion-like tube that contains sperm and is the site of oocyte fertilization. spermatheca   WBbt:0005319
Epidermal layer. hypodermis epidermis WBbt:0005733
type of cells that make up muscle layers in the pharynx. pharyngeal muscle cell   WBbt:0005451
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad posterior distal tip cell gon herm dtc P WBbt:0004506
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad anterior distal tip cell gon herm dtc A WBbt:0004520
neuron with its cell body situated in the head, excluding the pharynx. head neuron   WBbt:0006751
The organ in which the eggs are developed and protected until laid. uterus   WBbt:0006760
neuron with its cell body situated in the tail, posterior to rectum. tail neuron   WBbt:0006759
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite. P5.p hermaphrodite   WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite. P4.p hermaphrodite   WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite. P3.p hermaphrodite   WBbt:0008112
  P8.p hermaphrodite   WBbt:0008133
  P7.p hermaphrodite   WBbt:0008129
  P6.p hermaphrodite   WBbt:0008125
The tenth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P10   WBbt:0006779
The ninth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1. P9   WBbt:0006778
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1 P11 lineage name: ABplapappa WBbt:0004410
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1 P12 lineage name: ABprapappa WBbt:0004409
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar. vulF   WBbt:0006768
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr. vulE   WBbt:0006767
organ producing either sperm or ova. gonad   WBbt:0005175
Posterior daughter cell of P8.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P8.pp lineage name: P8.pp WBbt:0004145
Posterior daughter cell of P3.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P3.pp lineage name: P3.pp WBbt:0004153
Anterior daughter cell of P4.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P4.pa lineage name: P4.pa WBbt:0004151
Posterior daughter cell of P4.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P4.pp lineage name: P4.pp WBbt:0004149
Anterior daughter cell of P8.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P8.pa lineage name: P8.pa WBbt:0004147

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000908 daf-12 F11A1.3 Caenorhabditis elegans

1 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  All C. elegans development stages, including embryo, larva and adult stage. all stages Ce WBls:0000002