WormMine: ExpressionPattern Expr1917

• Pattern: In situ: Since multicopy transgene expression is silent in the C. elegans germline, Cecsb gene expression in the gonad was detected by in situ mRNA hybridization instead of GFP expression. The antisense cDNA probe of Cecsb stained the whole region of the gonad including oocytes, while the sense probe did not produce any signal. Reporter_gene Assay: GFP first appeared during the 50^100 cell stage and was detected throughout the embryonic development. GFP expression was also observed in all of the somatic cells up to the L3 larval stage. However, GFP expression was relatively stronger in pi and P lineage vulval cells at the L3 larval stage, where active cell division and dierentiation take place. At the L4 larval stage, GFP was relatively stronger in amphid and tail neurons, and vulva and somatic gonad cells than in other body regions. In adult hermaphrodites, the overall GFP expression weakened, but strong expression was still observed in intestine, head, and tail regions. In L4 and adult males, the tail region, including the spicule, protractor, and PC sensilla, showed intense fluorescence.
• Primary Identifier: Expr1917

11 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
female genital.	vulva		WBbt:0006748
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
neuron of the amphid sensillum	amphid neuron	amphid sensory neuron	WBbt:0005394
	copulatory spicule		WBbt:0005312
membranous cell attaches the uterus to the lateral epidermis (seam) and forms a thin laminar process dorsal to the vulva. formed by fusion of eight pi cell progeny and AC.	uterine seam cell	utse	WBbt:0006789
organ producing either sperm or ova.	gonad		WBbt:0005175
anterior-most body region containing the pharynx.	head		WBbt:0005739
	spicule protractor muscle		WBbt:0005826
a bilateral pair of sensilla immediately posterior of the cloaca.	postcloacal sensillum		WBbt:0005487

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000803	csb-1	F53H4.1	Caenorhabditis elegans

9 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010