WormMine: ExpressionPattern Expr8361

• Pattern: GFP expression initiated in the early gastrula. Robust expression of Prncs-1::GFP was observed in the midgut (E cell lineage) starting at the 28-cell stage and continuing into adulthood. By the comma stage, fluorescence was also visible in the embryo periphery in cells that give rise to hypodermis. In L1 larva and subsequent stages, strong expression of GFP was seen in hypodermal cells, including Hyp 7 syncytium and head and tail hypodermis. The expression pattern was identical in hermaphrodites and males, but adult hermaphrodites displayed fluorescence in vulval epithelium. Expression was absent in seam cells, nervous system, and pharynx. The Prncs-1::GFP reporter showed increased expression during starvation. Although fluorescence intensity was enhanced under starved conditions, the spatial expression pattern was unchanged. Expression of the Prncs-1::GFP transgene was also enhanced in males. An ~2.5-fold increase in rncs-1 expression in total RNA prepared from wild-type, well fed males, compared with hermaphrodites.
• Primary Identifier: Expr8361
• Remark: Picture: Figure 1.

13 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
Cylindrical hypodermal syncytium in head	hyp3	lineage name: ABplaapaaaa	WBbt:0004690
Cylindrical hypodermal syncytium in head	hyp4	lineage name: ABarpapapa	WBbt:0004687
Cylindrical hypodermal syncytium in head	hyp1	lineage name: ABalpaapppa	WBbt:0004694
Tail ventral hypodermis	hyp10	lineage name: ABplppppppp	WBbt:0004378
Tail ventral hypodermis, 8th hypodermis cell	hyp8		WBbt:0004381
Tail ventral hypodermis, 9th hypodermis cell	hyp9		WBbt:0004380
Cylindrical hypodermal syncytium in head	hyp2	lineage name: ABalpaapppp	WBbt:0004692
Tail dorsal hypodermis	hyp11	lineage name: Cpappv	WBbt:0004377
Cylindrical hypodermal syncytium in head	hyp5	lineage name: ABarpappap	WBbt:0004685
Preanal hypodermis	hyp12	lineage name: P12.pa	WBbt:0004376
Cylindrical hypodermal syncytium in head	hyp6	lineage name: ABarpaapaa	WBbt:0004679

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00023069	rncs-1	F55A4.9	Caenorhabditis elegans

10 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004