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Expr4594
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Analysis of a reporter transgene revealed that act-5 expression is restricted to a small subset of cells within the C. elegans alimentary tract and excretory systems. GFP fluorescence was easily detected within a subset of embryonic, larval, and adult cells in transgenic animals, consistent with previous findings on the act-5 expression pattern. Within larvae and adults, GFP expression occurred within the 20 cells that comprise the adult intestine, and in two sets of three associated cells: one just anterior and one posterior to the intestine. The anterior set corresponds to the middle of three sets of pharyngeal-intestinal valve cells (vpi2), whereas the posterior group of associated cells comprises the rectal epithelial cell (rectD, rect_VL, rect_VR). Finally, the single excretory canal cell, which extends processes along the entire length of the worm, also showed GFP expression. |
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Expr11809
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Robust GFP expression of pnc-1a::exon1a::GFP was detected in head and pharyngeal muscle, the two ASK neurons, the two distal tip cells and the three rectal gland cells (RectD, RectVL and RectVR). Whereas the four uv1 cells did not express pnc-1a::exon1a::GFP, four adjacent uv2 cells had moderate levels of GFP expression. Low levels of pnc-1a::exon1a::GFP expression were also detected in the vulval muscle. The pnc-1a reporter overlaps in expression pattern with the pnc-1b reporter only in head muscle and perhaps vulval muscle. |
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Expr963
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Transgenic animals bearing pFX1G1 had high levels of GFP fluorescence or immunoreactivity in embryonic and postembryonic neurons. fax-1::gfp expression was first detected in embryos prior to elongation (approximately 350 minutes of development). By approximately 400 minutes, there is strong fax-1::gfp expression in as many as 20 neurons in the embryonic head and 1-2 neurons in the embryonic tail. fax-1::gfp is expressed in 20 neurons postembryonically, through the adult stage. The position of these neurons indicates that most or all of them are among the 22 neurons that express fax-1::gfp embryonically. These cells include both AVKR and AVKL. fax-1::gfp was not observed in either of the HSN or PVQ neurons, or in the PVPR neuron at any stage of development. fax-1::gfp expression was observed in several other neurons and two non-neuronal cell types in transgenic animals carrying pFX1G1. These include the pairs of CEPD and URX sensory neurons, three pharyngeal neurons (M1, MI and probably M5), two pairs of ring interneurons (including the RIC pair), five neurons in the retrovesicular ganglion (including SABD and the pair of SABV neurons), a single neuron in the preanal ganglion (either PVPL or PVT) and a single neuron in the dorsorectal ganglion of the tail (probably DVA). There is incompletely penetrant fax-1::gfp expression in a few additional neurons that were not identified, and in the non neuronal dorsal rectal cell and distal tip cells of the somatic gonad. |
GFP immunoreactivity was present in the cytoplasm, axons and nuclei of cells. Axons of neurons that express fax-1::gfp embryonically were observed in the process of outgrowth. |
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Picture: Figure S4B to S4E. tbc-3 = F32B6.8 |
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Expr8101
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tbc-3 is not expressed in the somatic gonadal precursor cells (SGPs). Expression of tbc-3 was seen in seam cells, in pharynx and neurons in the head in the L2 stage. tbc-3 is also expressed in several cells in the tail region, which include rectal gland cells (the rectD cell). |
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Expr9959
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PDF-2::GFP signals could be observed throughout post-embryonic life. The PDF-2::GFP-expressing cells were identified as the interneurons BDUL/R, AVG, AIML/R, RIS, AVD and PVT, the chemosensory neuron pairs PHA and PHB, the motor neurons RID and RIML/R, the sensory neurons AQR and PQR, and less frequently in the PVPL/R interneurons. Outside the nervous system, strong expression was observed in rectal gland cells rectD and rectVL/R, the intestino-rectal valve cells virL/R and three posterior arcade cells in the head. Weak GFP fluorescence could also be observed in four additional head neurons that could not be unequivocally identified. GFP fluorescence was visible in neuronal cell bodies, axons and dendrites. |
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Because of the lack of nuclear or cytoplasmic expression, it was difficult to determine which cells were expressing egl-26::gfp in the animals carrying the translational fusion. Because pWH15 retained egl-26 activity as assayed by ability to rescue ku211 and ku228 mutants when expressed from an extrachromosomal array (2/4 lines rescued), it is assumed that the EGL-26::GFP expression pattern would accurately represent global and subcellular localization of EGL-26. |
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Expr1806
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Consistent with the results from the transcriptional fusion, the expression observed near the top of the vulval lumen in animals carrying the translational fusion corresponds most closely to the position of vulE. The 3-D expression pattern of the EGL-26::GFP translational fusion protein showed expression in a ring around the ventral region of the vulva, in a thicker region near the center of the vulva, and at the apex of the vulva corresponding to where the utse lies separating the vulval and uterine lumens. The thick region of expression in the center of the vulva closely corresponds to the vulE cells. The ring around the ventral region of the vulval most likely corresponds to expression by vulB2 as assayed by the transcriptional fusion. In animals that carry this fusion construct on an extrachromosomal array, kuEx90, expression was observed in many regions of the animal, although not ubiquitously. Expression is strong around the cells of the spermatheca, around the mouth, and lining the pharynx, the rectum, and the excretory canals. Expression was also seen in the pharyngeal intestinal junction cells, transiently during L3 in the anchor cell, in rectal epithelial cells D, VL, and VR, in B and in Y, and in several cells with a neuronal appearance. |
Expression is obvious near the vulva and the uterus only during L4. However, it is often very difficult to tell which cell is actually expressing egl-26::gfp because the fusion protein appears to line the lumen of the uterus and portions of the vulval lumen and is not obviously associated with any particular cell cytoplasm. Even in cases where a cell cytoplasm obviously contains egl-26::gfp, expression is often brighter around the apical edge of the cell. |
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Expr949
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gfp is expressed in a variety of cells, including the HSNs; hyp 8, 9, and 10; B; F; rect D; DVC; some ventral cord neurons; cells in the head; and cells in the preanal ganglion. sem-4 gfp is expressed in the VPCs. Expression in the VPCs is first observable during L2 and persists in the vulval lineages until the vulval divisions are complete. Expression is undetectable again by the mid-L4 stage, before vulval morphogenesis has terminated. Expression of sem-4 gfp in vulval cells is often strongest in the descendants of P7.p. |
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Expr9958
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PDF-1::GFP signals could be observed throughout post-embryonic life. pdf-1 reporter expression was found consistently in the interneurons ADAL/R and PVT, the chemosensory neuron pairs ASI, ASK, PHA and PHB, the RMED, RMEV and RID motor neurons, the sensory neuron pair ADE, the PQR mechanosensory neuron and less frequently in the PVPL/R interneurons. Strong non-neuronal expression could be detected in rectal gland cells rectD and rectVL/R, and the intestino-rectal valve cells virL/R. Faint GFP fluorescence appeared to be present in a small number of unidentified head neurons. GFP fluorescence was visible in neuronal cell bodies, axons and dendrites. |
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Expr8164
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Expressed in intestine: Exxx (20 intestine cells). Onset time: <200 cells. Expressed in pharynx: ABalpaxxx, ABaraaxxx, ABarapaxxx (57 pharyngeal cells, 4 neurons, 4 hypodermal cells). MSaaxxx, MSpaxxx (37 pharyngeal cells, 2 neurons, 10 muscle cells). Onset time: <200 cells. Expressed in rectal precursors: ABprpapppxx, ABprpppppax, ABplpppppax, ABplpapppxx (7 rectal and digestive muscle cells, 2 neurons, 1 muscle cell.) Onset time: >350 cells. |
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Picture: Figure S7, Movie 1. |
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Expr8105
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Expression patterns of ipla-1 in the adult stage. ipla-1 is expressed in several unidentified cells in pharynx (probably gland cells of pharynx), pharyngeal-intestinal valve, spermatheca, hypodermal syncytium, seam cells, rectal grand cells and intestinal-rectal valve. Expression patterns of ipla-1 in the larval stages. ipla-1 is expressed in intestine, three rectal gland cells, hypodermal syncytium and seam cells in the L1, L2 and L3 larva. In the L1 stage, ipla-1 expression is also observed in several unidentified cells in the tail. |
While IPLA-1 localizes to cytosol in intestine, spermatheca and gland cells, IPLA-1 localizes to the nuclei of hypodermal syncytium and seam cells. |
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Transgenic Marker: rol-6(su1006). |
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Expr517
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At approximately 260 min after the first cleavage, reporter gene expression is now also detected in nuclei on the dorsal surface of the embryo, corresponding to the position of the major hypodermal cells, and in six nuclei on each ventrolateral aspect of the embryo, corresponding to the position of the ventral hypodermal cells (P cells). Expression was first seen in granddaughters of C.paa and C.aaa about 240 min. after first cleavage. About 260 min. after first cleavage, expression was seen in dorsal and ventral hypodermal cells. Between comma-stage and 3-fold stage, expression was seen in dorsal and ventral hypodermal cells, and in tail and head hypodermis. After the 3-fold stage, expression becomes reduced, but is maintained a low levels in larvae and adults. After the 3-fold stage through adulthood, a digestive-tract component is expressed: probably vpi3D and vpi3V in the pharyngeal intestinal valve, virL and virR in intestinal rectal valve, and rect D, rect VL, rect VR. |
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Reporter gene fusion type not specified. |
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Expr2725
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Expressed in rectal epithelial cells RepD, RepVL and RepVR. Not expressed in intestino-rectal valve, K, K', U, R, B or Y cells. |
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Reporter gene fusion type not specified. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr633
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Two different grd-1 constructs, an amino-terminal grd-1M::gfp and a grd-1C::gfp-lacZ reporter construct are expressed in the rectal cells of larvae to adult, possibly rectal epithelial cells. grd-1M::gfp is expressed the rectal epithelium cells rect D, rect VL, and rect VR. |
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