WormMine: ExpressionPattern Expr12085

• Pattern: plr-1::GFP expression became visible in late gastrulation stage embryos. Strongest expression was detected in many cells in the tail region and by comma stage the most prominent expression was seen in body wall muscle cells. In early larval stages expression was detectable in a number of different tissues including the major hypodermal cells, muscle and marginal cells of the pharynx, the intestine (strongest in the anteriormost and posteriormost cells) as well as the anal depressor and stomatointestinal muscle. In the nervous system GFP expression was visible in a few neurons in head ganglia, many ventral cord motor neurons and several neurons in the tail ganglia including the PDA neuron. Based on the lack of commissures the motor neurons are likely of the VA, VB, VC and/or AS class. Based on the number of cells the majority (or even all) of these classes express GFP. In general expression was strongest in the tail region throughout development. This also held true for the motor neurons in the ventral cord, where GFP was strongest in the posterior-most cells and barely detectable in anterior motor neurons. Expression is maintained throughout larval development. In later larval stages expression is also seen occasionally in the distal tip cell of the developing gonad, the vulva and uterine muscle cells and the VC4 and VC5 neurons flanking the vulva. Expression in AVG, HSN or CAN neurons was not detectable with this reporter construct, but was detected in a previous study in HSN and CAN using a longer genomic construct (Moffat et al., 2014, Expr11480).
• Primary Identifier: Expr12085

20 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
female genital.	vulva		WBbt:0006748
somatic cell that situates at the tip of a gonad arm.	distal tip cell	DTC	WBbt:0006865
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.	anal depressor muscle	dilator muscle	WBbt:0004292
Neuron class of eleven motoneurons, distributed along the ventral cord, which innervate ventral body muscles.	VB neuron		WBbt:0005336
Neuron class of twelve motoneurons, distributed along the ventral cord, which innervate ventral body muscles.	VA neuron		WBbt:0005339
Neuron class of eleven motoneurons, with cell bodies in the ventral cord, which innervate dorsal muscles.	AS neuron		WBbt:0005334
Neuron class of six motoneurons in the ventral cord, which innervate ventral body muscles and the vm2 muscles of the vulva.	VC neuron		WBbt:0005304
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC4	lineage name: P6.aap	WBbt:0004617
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC5	lineage name: P7.aap	WBbt:0004613
intestinal muscle cell, attach to intestine and body wall anterior to anus	intestinal muscle	stomato-intestinal muscle	WBbt:0005796
posterior region, from rectum to the end	tail		WBbt:0005741
muscle lining of the uterine wall.	uterine muscle		WBbt:0005342
ganglion in the head.	head ganglion		WBbt:0005135
a type of pharyngeal cell that connects basement membrane to the apices of triangle-shaped pharyngeal lumen.	marginal cell		WBbt:0003673
Neuron class of one motor neuron, process in dorsal cord, same as Y cell in hermaphrodite, Y.a in male.	PDA		WBbt:0004386
ganglion in the tail region (posterior to rectum).	tail ganglion		WBbt:0006977

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00023404	plr-1	Y47D3B.11	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010