WormMine: ExpressionPattern Expr16049

• Pattern: nmgp-1 expresses mostly in sensory neurons and in the egg-laying apparatus of adult hermaphrodites. GFP expression driven by the putative nmgp-1 promoter was detected in several cells, primarily neurons. Within the head, we saw GFP expression in pharyngeal neurons, as many somas and processes within the metacorpus and the posterior bulb ventral ganglia were observed. In addition, some of the processes next to the bulb might correspond to CEP sheath glial cells. In the midbody, we saw labeling of cells within the egg-laying apparatus. Posteriorly, we observed cells in the tail ganglia and possibly phasmid neurons. Neuronal processes along dorsal and ventral cords were also labeled. In addition, to identify specific neurons, we used the NeuroPAL strain (OH15500) developed by Hobert's Lab (Yemini et al., 2021). This strain has a stereotyped fluorescent color map to identify all neurons. We injected it with the same plasmid for GFP expression under the nmgp-1 promoter. The following neurons were identified as expressing GFP: ALA, CEPD, IL1 (head neurons from the nerve ring), the sensory amphid neurons ASK, neurons from the anterior ventral nerve cord (VA6, VB7, DB5, AS5, VD6, DD3, DA4) and posterior ventral cord (VA11, VD11, AS10, DA7, DB7, CB11, VA11), neurons from the preanal ganglion (PVP, PVT, DD6, AS11, VA12, DA8, DA9) dorso-rectal ganglion (DVB, DVA, DVC) and lumbar ganglion (PVQ, PHC). The neurons identified include sensory neurons (amphid and mechanosensory), motor neurons and interneurons.
• Primary Identifier: Expr16049

54 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
Neuron class of one interneuron, projects along ventral cord to ring.	PVT	lineage name: ABplpappppa	WBbt:0004070
neuron of the pharyngeal nervous system.	pharyngeal neuron		WBbt:0005439
Inner labial neuron	IL1VR	lineage name: ABarapppppaa	WBbt:0004547
Inner labial neuron	IL1DR	lineage name: ABalappppaaa	WBbt:0004737
Inner labial neuron	IL1L	lineage name: ABalapaappaa	WBbt:0004553
Inner labial neuron	IL1R	lineage name: ABalaappppaa	WBbt:0004551
Inner labial neuron	IL1VL	lineage name: ABalppapppaa	WBbt:0004549
Inner labial neuron	IL1DL	lineage name: ABalapappaaa	WBbt:0004738
neuron making synapse on an effector (muscle, gland).	motor neuron	motoneuron	WBbt:0005409
a bundle of nerve processes that runs along the dorsal mid-line of the animal.	dorsal nerve cord	dorsal cord	WBbt:0006750
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASKL	lineage name: ABalpppapppa	WBbt:0003884
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASKR	lineage name: ABpraaaapppa	WBbt:0003883
Interneuron, projects along ventral cord to ring	PVQL	lineage name: ABplapppaaa	WBbt:0004076
Interneuron, projects along ventral cord to ring	PVQR	lineage name: ABprapppaaa	WBbt:0004074
Neuron class of one neuron, sends processes laterally and along dorsal cord.	ALA	lineage name: ABalapppaaa	WBbt:0003955
neuron of phasmid sensillum	phasmid neuron		WBbt:0006753
neuron of the amphid sensillum	amphid neuron	amphid sensory neuron	WBbt:0005394
Ventral cord motor neuron, innervates ventral body muscles	VB7	lineage name: P6.aaap	WBbt:0004631
neuron that senses external stimuli and transduce them to signals internal to the organism.	sensory neuron		WBbt:0005759
the ganglion that lies above and behind the rectum in the tail, in close continuity with the anal hypodermal ridge. It contains 3 neuron cell bodies (DVA, DVB and DVC) that send their neuronal processes into the ventral nerve cord via dorso-rectal commissures that encircle the anus. The ganglion contains no local neuropil in the hermaphrodite. In the adult male tail, this ganglion gains additional neurons and some local neuropil.	dorso-rectal ganglion		WBbt:0005212
The left and right lumbar ganglia lie behind the pre-anal ganglion in the tail, in lateral positions. Each contains 12 neuron cell bodies that send most of their neuronal processes into the ventral nerve cord via lumbar commissures and the pre-anal ganglion. There is no local neuropil in these ganglia in the hermaphrodite, instead they form most synapses in the pre-anal ganglion. Most lumbar neurons have sensory functions. In the adult male tail, these two ganglia gain many additional neurons and have some local neuropil. The lumbar neurons lie in close association with the lateral hypodermis, and most are posterior to the anus.	lumbar ganglion	lumbar lateral ganglia	WBbt:0005830
Ventral cord motor neurons, innervate dorsal muscles	DB5	lineage name: ABplpapappp	WBbt:0004842
Ventral cord motor neurons, innervate dorsal muscles	DB7	lineage name: ABprppaappp	WBbt:0004840
Ventral cord motor neurons, innervate dorsal muscles	DA7	lineage name: ABprpppaaap	WBbt:0004859
Ventral cord motor neurons, innervate dorsal muscles	DA4	lineage name: ABplppapapp	WBbt:0004865
Cephalic neurons, contain dopamine	CEPDL	lineage name: ABplaaaaappa	WBbt:0004938
Cephalic neurons, contain dopamine	CEPDR	lineage name: ABarpapaappa	WBbt:0004937
Neuron class of one ring interneuron, cell body in dorsorectal ganglion, has a large vesicle-filled process in ring.	DVA	lineage name: ABprppppapp	WBbt:0004823
Neuron class of one ring interneuron, cell body in dorsorectal ganglion, inervates rectal muscles.	DVB	lineage name: K.p	WBbt:0004822

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00017437	nmgp-1	F13H8.4	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage after an hermaphrodite animal is fully-developed and reaches maturity.		adult hermaphrodite Ce	WBls:0000057