WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00003936 Gene Name  pat-12
Sequence Name  ? T17H7.4 Brief Description  pat-12 encodes several isoforms containing a novel hemidesmosome component that has strong homology to antigen B20 from filarial nematode onchocerca volvulus, which partly protects different hosts against infection when used for immunization; PAT-12 activity is important to maintain FO integrity and might have distinct functions apically and basally based on differential loss of VAB-10A, CIFs and ECM receptors at the two plasma membrane; in pat-12 mutants muscles reach their normal position but fail to maintain them once they start to contract suggesting CeHDs are defective; epidermal integrity is lost in pat-12 mutants resulting from internal rupture; PAT-12A localization depends another fibrous organelle components; PAT-12 function is dispensable for feeding, fertilization and egg-laying; pat-12 expression is complex with isoforms A, E, J, K being fibrous organelle components: the isoforms A and J are in pharyngeal and epidermal FOs; during late embryogenesis, PAT-12K colocalizes with junctions between seam cells and epidermal cells, PAT-12E/F/I is detected in spermatheca plasma membrane, weakly in vulva; are present where cIFs are located.
Organism  Caenorhabditis elegans Automated Description  Involved in embryonic morphogenesis and hemidesmosome assembly. Located in hemidesmosome. Expressed in head neurons; mechanosensory neurons; rectum; seam cell; and spermatheca.
Biotype  SO:0001217 Genetic Position  III :-26.2422 ±0.011603
Length (nt)  ? 25220
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00003936

Genomics

19 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:T17H7.4b.1 T17H7.4b.1 2244   III: 727207-746538
Transcript:T17H7.4e.1 T17H7.4e.1 3659   III: 727207-734120
Transcript:T17H7.4d.1 T17H7.4d.1 5130   III: 727208-738978
Transcript:T17H7.4k.2 T17H7.4k.2 2408   III: 727208-743317
Transcript:T17H7.4k.1 T17H7.4k.1 2434   III: 727208-743343
Transcript:T17H7.4g.2 T17H7.4g.2 2342   III: 727208-743344
Transcript:T17H7.4j.1 T17H7.4j.1 2608   III: 727208-752426
Transcript:T17H7.4a.1 T17H7.4a.1 2424   III: 727211-746542
Transcript:T17H7.4g.1 T17H7.4g.1 2426   III: 727215-746693
Transcript:T17H7.4d.2 T17H7.4d.2 5135   III: 727296-738978
Transcript:T17H7.4i.1 T17H7.4i.1 1736   III: 727328-732258
Transcript:T17H7.4h.1 T17H7.4h.1 1373   III: 727639-732335
Transcript:T17H7.4c.1 T17H7.4c.1 2121   III: 727639-746538
Transcript:T17H7.4d.3 T17H7.4d.3 4182   III: 728363-738978
Transcript:T17H7.4d.4 T17H7.4d.4 4041   III: 728363-738978
Transcript:T17H7.4m.1 T17H7.4m.1 66   III: 730602-730667
Transcript:T17H7.4d.5 T17H7.4d.5 3369   III: 730635-738978
Transcript:T17H7.4d.6 T17H7.4d.6 3438   III: 731931-738978
Transcript:T17H7.4d.7 T17H7.4d.7 3299   III: 732147-738978
 

Other

11 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:T17H7.4e T17H7.4e 1431   III: 727639-727705
CDS:T17H7.4i T17H7.4i 1344   III: 727639-727705
CDS:T17H7.4j T17H7.4j 2151   III: 727639-727705
CDS:T17H7.4k T17H7.4k 1974   III: 727639-727705
CDS:T17H7.4d T17H7.4d 3093   III: 732353-732532
CDS:T17H7.4a T17H7.4a 1992   III: 727639-727705
CDS:T17H7.4b T17H7.4b 1812   III: 727639-727705
CDS:T17H7.4c T17H7.4c 2121   III: 727639-727705
CDS:T17H7.4g T17H7.4g 1128   III: 727639-727705
CDS:T17H7.4h T17H7.4h 1215   III: 727639-727705
CDS:T17H7.4m T17H7.4m 66   III: 730602-730667

14 RNAi Result

WormBase ID
WBRNAi00053461
WBRNAi00053462
WBRNAi00053463
WBRNAi00066812
WBRNAi00009181
WBRNAi00024291
WBRNAi00006590
WBRNAi00002646
WBRNAi00035633
WBRNAi00062896
WBRNAi00007058
WBRNAi00062901
WBRNAi00077521
WBRNAi00026303

564 Allele

Public Name
gk963623
gk962532
gk964281
WBVar01322303
WBVar01322305
WBVar01322304
WBVar01322309
WBVar01322308
st430
WBVar02069520
WBVar02069521
WBVar02069512
WBVar02069513
WBVar02069514
WBVar02069515
WBVar02069516
WBVar02069517
WBVar02069518
WBVar02069519
WBVar01540520
WBVar00031523
WBVar00031538
WBVar00031533
WBVar00031543
WBVar00031513
WBVar00031448
WBVar00031443
WBVar00031453
WBVar00031473
WBVar00031478

1 Chromosome

WormBase ID Organism Length (nt)
III Caenorhabditis elegans 13783801  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00003936 727207 752426 -1

3 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  

1 Downstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrIII_726156..727206   1051 III: 726156-727206 Caenorhabditis elegans

215 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Transcripts of coding genes that showed significantly decreased expression in muscle. DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed. WBPaper00062325:muscle_depleted_coding-RNA
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
  mRNAs that showed decreased expression in 1 cell mebryo comparing to in oocyte, according to RNAseq analysis. Gaussian error propagation. As cutoff for the up-regulated genes authors used log2 fold change > 1 and P < 0.05 and as cutoff for the down-regulated genes authors used log2 fold change < -1 and P < 0.05. WBPaper00045420:fertilization_downregulated_transcript
  TGF- Dauer pathway adult transcriptional targets. Results obtained by comparing the microarray results of the dauer-constitutive mutants daf-7(e1372), daf-7(m62), and daf-1(m40) with dauer-defective mutants daf-3(mgDf90), daf-5(e1386), and daf-7(e1372);daf-3(mgDf90) double mutants at the permissive temperature, 20C, on the first day of adulthood. SAM WBPaper00031040:TGF-beta_adult_upregulated
Bacteria: E.faecalis strain OG1RF Transcripts that showed significantly increased expression after infection by E. faecalis OG1RF. Ballgown was used to calculate differential expression of genes using FPKM data and to generate tables with fold change and P values. Genes were shortlisted with a cutoff of 2-fold change and P values of less than 0.05. WBPaper00059754:E.faecalis_OG1RF_upregulated
  Transcripts that showed significantly increased expression glp-1(e2141); TU3401 animals comparing to in TU3401 animals. Fold change > 2, FDR < 0.01. WBPaper00065993:glp-1(e2141)_upregulated
  Genes that were downregulated in lin-15B(n744). For each gene in each microarray hybridization experiment, the ratio of RNA levels from the two samples was transformed into a log2 value and the mean log2 ratio was calculated. The log2 ratios were normalized by print-tip Loess normalization (Dudoit and Yang, 2002). All genes with a false discovery rate of <= 5% (q <= 0.05) (Storey and Tibshirani, 2003) and a mean fold-change ratio of >= 1.5 were selected for further analysis. WBPaper00038168:lin-15B(n744)_downregulated
Bacteria infection: Enterococcus faecalis Genes with increased expression after 24 hours of infection by E.faecalis Fold changes shown are pathogen vs OP50. For RNA-seq and tiling arrays, log2 fold changes between gene expression values of infected versus uninfected nematodes were calculated. For log2 fold changes > 0.00001 the values > 81.25th percentile were defined as up-regulated and for log2 fold changes < -0.00001 the values < 18.75th percentile were defined as down-regulated. WBPaper00038438:E.faecalis_24hr_upregulated_TilingArray
  Coexpression clique No. 60, 176662_at-Y53F4B.16, on the genome-wide coexpression clique map for the nematode GPL200 platform. All available microarray datasets for the GPL200 platform (Affymetrix C. elegans Genome Array) were obtained from the GEO repository. This included 2243 individual microarray experiments. These were normalized against each other with the software RMAexpress (Bolstad, 2014). Based on these normalized values, Pearsons correlation coefficients were obtained for each probe-probe pair of the 22,620 probes represented on this array type. The resulting list of correlation coefficients was then ranked to generate the ranked coexpression database with information on each probe represented on the GPL200 platform. WBPaper00061527:176662_at-Y53F4B.16
  Proteins interacting with NHR-49-GFP according to co-IP and LC-MS. N.A. WBPaper00064071:NHR-49_interacting
  Transcripts expressed in the epithelial tissues surrounding the pharynx that includes the arcade and intestinal valve (AIV) cells, according to PAT-Seq analysis using Pbath-15-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:arcade_intestinal-valve_expressed
  Transcripts expressed in body muscle, according to PAT-Seq analysis using Pmyo-3-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:body-muscle_expressed
  Transcripts that showed significantly decreased expression at 5-days-post L4 adult N2 hermaphrodites comparing to 1-day-post L4 adult N2 hermaphrodites. DESeq2, fold change > 2, FDR < 0.05 WBPaper00065835:Day5_vs_Day1_downregulated
  Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:GABAergic-neuron_expressed
  Transcripts that showed significantly decreased expression at 11-days-post L4 adult N2 hermaphrodites comparing to 1-day-post L4 adult N2 hermaphrodites. DESeq2, fold change > 2, FDR < 0.05 WBPaper00065835:Day11_vs_Day1_downregulated
  Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:hypodermis_expressed
  Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:intestine_expressed
  Transcripts expressed in pharynx, according to PAT-Seq analysis using Pmyo-2-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:pharynx_expressed
  Genes with expression level regulated by genotype (N2 vs CB4856) and age at old adults stage (214 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_age_regulated_aging
  Genes with expression level regulated by genotype (N2 vs CB4856) and age at L3 larva and Late reproduction stage (96 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_age_regulated_developing
  Transcripts that showed significantly increased expression in day 1 adult hermaphrodite comparing to in L4 larva daf-16(mu86);glp-1(e2141) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-1-adult_vs_L4_upregulated_daf-16(mu86);glp-1(e2141)
  Genes with expression level regulated by genotype (N2 vs CB4856) at old adults stage (214 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_regulated_aging
  Genes with expression level regulated by genotype (N2 vs CB4856) at L3 larva and Late reproduction stage (96 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_regulated_developing
Bacteria infection: Bacillus thuringiensis Transcripts that showed significantly increased expression in N2 animals infected by bacteria BMB171/Cry5Ba, an acrystalliferous Bt mutant BMB171 transformed with toxin gene cry5Ba on the shuttle vector pHT304, comparing to N2 animals infected by BMB171/pHT304. N.A. WBPaper00064229:B.thuringiensis-Cry5Ba_upregulated
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 10 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.1mix_downregulated_12h
  Transcripts that showed significantly increased expression after four-day-old young adult worms were placed on NGM plates seeded with OP50 in the presence 5% Agaro-oligosaccharides(AGO) for 24 h, comparing to animals grown in the absence of AGO. Fold change > 2. WBPaper00064306:Agaro-oligosaccharides_upregulated
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 6h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.5mix_downregulated_6h
  Transcripts that showed significantly increased expression in sin-3(tm1276) comparing to in N2. DESeq2, fold change > 2, p-value < 0.01. WBPaper00061203:sin-3(tm1276)_upregulated
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.5mix_downregulated_12h
  Transcripts that showed significantly increased expression in mrg-1(qa6200) comparing to in control animals in primordial germ cells (PGCs) at L1 larva stage. DESeq2(v1.32.0), FDR < 0.05. WBPaper00064315:mrg-1(qa6200)_upregulated_PGCs

10 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr1031868 Tiling arrays expression graphs  
Strain: BC14887 [gei-16::gfp] transcriptional fusion. PCR products were amplified using primer A: 5' [GCGCAGTTTTGGTTTTTGA] 3' and primer B 5' [CAATGTGCGAAGTGATCGTC] 3'. Expr6705 Adult Expression: rectal epithelium; Reproductive System; vulva other; hypodermis; Larval Expression: rectal epithelium; Reproductive System; developing vulva; developing uterus; hypodermis;  
    Expr11569 Isoform gei-16J had a distribution characteristic of pharyngeal fibrous organelles. Specifically, PAT-12J was present at the basal and apical plasma membranes of the pharynx, like the plakin VAB-10A. In addition, PAT-12J::GFP appeared to be expressed in a punctate manner that might indicate co-localization with cIFs or aggregation.  
Original chronogram file: chronogram.1023.xml [T17H7.4:gfp] transcriptional fusion. Chronogram29    
Temporal description   Expr11568 Isoform gei-16A had a distribution characteristic of epidermal fibrous organelles. Specifically, PAT-12A colocalized with cytoplasmic intermediate filaments (cIFs) in the epidermis.  
Temporal description   Expr11570   During late stages of embryogenesis, PAT-12K colocalized with junctions between seam and other epidermal cells, and formed circumferentially oriented bands in seam cells. As revealed by co-expressing PAT-12K::GFP with the actin-binding reporter VAB-10ABD::mCherry, this bundle pattern appeared to partially coincide with actin,although the circumferentially oriented seam microfilament bundles highlighted by mCherry started to fade away before overlapping the PAT-12K::GFP signal. Thus, the nature of the PAT-12K filaments remains uncertain. In larvae, PAT-12Kdistribution was quite similar to that of PAT-12A in the epidermis, though much fainter.
    Expr11571 The isoforms PAT-12E/F/I were detected in the spermatheca, where they marked the plasma membrane, and very weakly in the vulva.  
    Expr2032983 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr1157027 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  
    Expr2014749 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  

24 GO Annotation

Annotation Extension Qualifier
  located_in
  located_in
  located_in
  located_in
  involved_in
  involved_in
  enables
  enables
  located_in
  located_in
  located_in
  located_in
  located_in
  located_in
  located_in
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables

0 Homologues

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00003936 727207 752426 -1

24 Ontology Annotations

Annotation Extension Qualifier
  located_in
  located_in
  located_in
  located_in
  involved_in
  involved_in
  enables
  enables
  located_in
  located_in
  located_in
  located_in
  located_in
  located_in
  located_in
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables
  enables

0 Regulates Expr Cluster

1 Sequence

Length
25220

1 Sequence Ontology Term

Identifier Name Description
gene  

2 Strains

WormBase ID
WBStrain00033497
WBStrain00035847

1 Upstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrIII_752427..755261   2835 III: 752427-755261 Caenorhabditis elegans