WormMine: ExpressionPattern Expr2707

• Pattern: Expression using the upstream regulatory regions of exon 1bc (ser-2prom2::gfp) is mostly restricted to the AIYL/R, AIZL/R, RID, DVA, BDUL/R, SIADL/R, and SIAVL/R interneurons. Less consistent expression is observed in PVT. In addition, expression is observed in the RMEL/R motor neurons. Outside the nervous system, expression can be observed in the excretory gland cells. No more transcriptional regulatory information is contained within intronic regions by generating a fusion of gfp to the full coding genomic ser-2 locus using an in vivo recombination technique([ser-2::gfp] translational fusion. Transgenic animals expressing such a construct show an expression pattern similar to the one observed with the ser-2prom1::gfp construct. The upstream regulatory region of the third splice form, containing exon 1d(ser-2prom3::gfp), drives expression exclusively in two sensory neuron classes, OLL(L/R) and PVD(L/R). ser-2prom1::gfp is expressed in the AIY interneuron class and a set of unidentified neurons. These neurons were identified as head and tail interneuron classes, namely AVHL/R, AUAL/R, AIYL/R, RICL/R, SABVL/R, RID, RIAL/R, SABD, SDQ, CANL/R, DA9, LUAL/R, ALNL/R, and PVCL/R. In addition to its expression in neurons, ser-2prom1::gfp is also expressed in pharyngeal cells (NSM neurons and pm1/6 muscles) and in head muscles. In males, expression can be observed in posterior dorsal and ventral body wall muscles, the male-specific diagonal muscles, and several posterior neurons likely to be CP neurons.
• Primary Identifier: Expr2707
• Reporter Gene: [ser-2::gfp] transcriptional fusion constructs. Ser-2 reporter constructs were generated by using a PCR fusion protocol, using pPD95.75 as a template for green fluorescent protein (gfp). For all gfp fusion primers listed, gfp vector sequence is indicated in lowercase, and gene-specific sequence is indicated in uppercase. [ser-2::gfp] translational fusion. A translational fusion of the whole ser-2 locus to gfp was created by using an in vivo recombination technique. Specifically, two overlapping PCR fragments, one containing the 5' part of a locus, the other containing the remainder of the locus PCR-fused to gfp, were coinjected into the worm. Recombination of these two fragments via the homologous region leads to the expression of a full-length ser-2::gfp fusion.

49 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Neuron class of one interneuron, projects along ventral cord to ring.	PVT	lineage name: ABplpappppa	WBbt:0004070
gland cell of the secretory-excretory system, sends processes to ring, opens into excretory duct.	excretory gland cell	exc gl	WBbt:0005776
Amphid interneuron	AIYL	lineage name: ABplpapaaap	WBbt:0003963
Amphid interneuron	AIYR	lineage name: ABprpapaaap	WBbt:0003961
Pharyngeal neurosecretory motorneuron, contain serotonin	NSML	lineage name: ABaraapapaav	WBbt:0004457
Pharyngeal neurosecretory motorneuron, contain serotonin	NSMR	lineage name: ABaraapppaav	WBbt:0004446
Ring interneuron	RICL	lineage name: ABplppaaaapp	WBbt:0003941
Ring interneuron	RICR	lineage name: ABprppaaaapp	WBbt:0003940
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCL	lineage name: ABplpppaapaa	WBbt:0004094
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCR	lineage name: ABprpppaapaa	WBbt:0004092
Amphid interneuron	AIZL	lineage name: ABplapaaapav	WBbt:0003959
Amphid interneuron	AIZR	lineage name: ABprapaaapav	WBbt:0003957
first layer pharyngeal muscle cell.	pm1	M1	WBbt:0003744
Neuron class of one ring motoneuron, projects along dorsal cord, innervates dorsal body muscle.	RID	lineage name: ABalappaapa	WBbt:0003938
Process runs along excretory canal, no synapses, essential for survival	CANL	lineage name: ABalapaaapa	WBbt:0004949
Process runs along excretory canal, no synapses, essential for survival	CANR	lineage name: ABalappappa	WBbt:0004947
Ant. lateral interneuron, process projects into ring	SDQR	lineage name: QR.pap	WBbt:0004991
Neuron, process runs along excretory canal and into ring, unique darkly staining synaptic vesicles	BDUL	lineage name: ABarppaappp	WBbt:0003812
Neuron, process runs along excretory canal and into ring, unique darkly staining synaptic vesicles	BDUR	lineage name: ABarpppappp	WBbt:0003811
Neuron class of one ring interneuron, cell body in dorsorectal ganglion, has a large vesicle-filled process in ring.	DVA	lineage name: ABprppppapp	WBbt:0004823
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIAVL	lineage name: ABplpapappa	WBbt:0004985
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIAVR	lineage name: ABprpapappa	WBbt:0004983
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIADR	lineage name: ABprpapaapa	WBbt:0004987
Receives a few synapses in the ring, has a posteriorly directed process that runs sublaterally	SIADL	lineage name: ABplpapaapa	WBbt:0004989
Ring motor neuron	RMER	lineage name: ABalaaaarrp	WBbt:0005023
Ring motor neuron	RMEL	lineage name: ABalaaaarlp	WBbt:0005025
Neuron, lateral process adjacent to excretory canal	PVDL	lineage name: V5L.paapa	WBbt:0004090
Neuron, lateral process adjacent to excretory canal	PVDR	lineage name: V5R.paapa	WBbt:0004088
Lateral outer labial neurons	OLLL	lineage name: ABalppppapaa	WBbt:0004445

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004777	ser-2	C02D4.2	Caenorhabditis elegans

0 Life Stages